Dynamic Testing of Signal Transduction Deregulation During Breast Cancer Initiation
Abstract
The goal of our study is to develop nanosensors to detect pre-cancerous changes in the breast and figure out which pre-cancerous breast changes have the highest chance of progressing to become invasive breast cancer. In particular, we are developing targeted nanobiosensors to test real-time protein phosphorylation signaling in live RPFNA cells. In this study, we have designed fiber-optic nanoprobes for optical detection in single living cells. The nanoprobes were fabricated with well-controlled nanoapertures for optimized spatial resolution and optical transmission. The nanoprobes were characterized with scanning electron microscopy and near-field scanning optical microscopy to ensure optimal performance. Theoretical analysis determined the probing depth and volume of the nanoprobe. The nanoprobes have been demonstrated for detection of biologically significant species in single living cells. The nanobiosensors allow us to directly test for complex protein changes in breast cells taken from high-risk women.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2011
- Accession Number
- ADA549536
Entities
People
- Anuj Dhawan
- Benoit Lauly
- Hsiang-kuo Yuan
- Hsinneng Wang
- Jonathan P. Scaffidi
- Molly K. Gregas
- Stephen De Vience
- Tuan Vo-Dinh
- Victoria Seewaldt
- Yan Zhang
Organizations
- Duke University