Induction and Acceleration of Mammary Tumors by Activated P13 Kinase
Abstract
During this time period the necessary retroviral vectors were developed, used to transduce mammary epithelial cells, and these transduced cells were transferred into the cleared fat pad of recipient mice. Although ductal outgrowth occurred in the cleared fat pads indicating that the transplant was successful, the number of GFP positive ducts that grew out was very low. This was surprising given that a high proportion (greater than 80%) of the mammary epithelial cels were GFP-positive after infection. It is not clear whether the engraftment of the transduced cells was poor, of the outgrowth of these cells was diminished due to high expression of the transgene. Further studies related to these points were curtained due to the departure of the graduate student from graduate school.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2009
- Accession Number
- ADA549644
Entities
People
- Erica Nolte
- Steve Anderson
Organizations
- University of Colorado Health