Isolation, Expression Analysis, and Functional Characterization of the First Antidiuretic Hormone Receptor in Insects

Abstract

Diuresis following blood-gorging in Rhodnius prolixus is the major process leading to the transmission of Chagas disease. We have cloned the cDNA of the first receptor known to be involved in an antidiuretic strategy in insects, a strategy that prevents diuresis. This receptor belongs to the insect CAPA receptor family known in other insects to be activated by peptides encoded within the capability gene. We characterize the expression profile in fifthinstars and find expression is localized to the alimentary canal. Highest transcript levels are found in Malpighian tubules and the anterior midgut, which are known targets of the antidiuretic hormone, RhoprCAPA- 2.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2010
Accession Number
ADA549706

Entities

People

  • Ian Orchard
  • Jean-paul Paluzzi
  • Ronald J. Nachman
  • Yoonseong Park

Organizations

  • United States Department of Agriculture

Tags

Communities of Interest

  • Ground and Sea Platforms

DTIC Thesaurus Topics

  • Acids
  • Amines
  • Amino Acids
  • Animal Structures
  • Cell Line
  • Cells
  • Central Nervous System
  • Disease Vectors
  • Drosophila
  • Genetic Code
  • Genetic Structures
  • Genetics
  • Health Services
  • Identification
  • Nervous System
  • Nucleic Acids
  • Pest Control

Readers

  • Cardiovascular Physiology
  • Molecular Genetics
  • Vector-Borne Disease and Entomology