Examination of a Newly Discovered Human Retrovirus, XMRV, in Breast Cancer

Abstract

The main objective of this research project is to test the hypothesis that a newly discovered retrovirus, XMRV, is involved development of human breast cancer. To accomplish this, XMRV protein and nucleic acid will be detected in breast cancer specimens that have been formalin-fixed and paraffin-embedded (FF-PE). We have obtained the required number of FF-PE breast specimens for this study from Asterand, Inc., Detroit, MI. We have optimized assay conditions for the detection of XMRV protein PE sections by the identification of an antibody that is at least four-fold more sensitive than other antibodies that have been previous studies. This was done by our production of cell lines that express infectious XMRV virus by starting with plasmid containing the cloned XMRV genome. With this antibody we have been able to detect XMRV protein by immunohistochemistry analysis of these cell lines. These results will thus enable us to carry out the IHC analysis of the FF-PE breast cancer specimens.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2011
Accession Number
ADA549919

Entities

People

  • Fayth K. Yoshimura

Organizations

  • Wayne State University

Tags

DTIC Thesaurus Topics

  • Acids
  • Alkanes
  • Antibodies
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Cultured Cells
  • Detection
  • Diseases And Disorders
  • Identification
  • Immunohistochemistry
  • Infection
  • Neoplasms
  • Nucleic Acids
  • Prostate Cancer
  • Proteins

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Molecular and genetic basis of cancer.
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology