Notch in Pathological Angiogenesis and Lymphangiogenesis

Abstract

The purpose of this study was to determine the role of Notch signaling in lymphatic endothelial cell (LEC) behavior and to determine the effects on tumor vasculature upon Notch inhibition. We hypothesized that inhibiting Notch activity may disrupt tumor (lymph)angiogenesis by changing expression and activity of EC genes. To that end, we have created a treatment agent known as Notch1 decoy (hN1DFc). Activation of Notch changes the gene profile of LEC and changes their in vitro behavior. An orthotopic model of human breast cancer was established. Pilot studies show that expression by these tumors of a mutant VEGF-C induces tumor lymphangiogenesis and lymph node metastasis. These tumors are rich in Notch-positive vasculature, making this a good model for future studies of Notch inhibition on tumor vasculature.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2011
Accession Number
ADA550314

Entities

People

  • Minji Kim

Organizations

  • Columbia University

Tags

DTIC Thesaurus Topics

  • Angiogenesis
  • Biomedical Research
  • Blood
  • Blood Vessels
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Cellular Structures
  • Department Of Defense
  • Endothelial Cells
  • Inhibition
  • Lymph Nodes
  • Lymphatic System
  • Lymphatic Vessels
  • Neoplasms
  • Pilot Studies

Fields of Study

  • Biology
  • Medicine

Readers

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