Reactivation of Breast Cancer Micrometastases by Senescent Bone Marrow Stroma

Abstract

We developed in vitro injury model of stroma cultured from bone marrow samples from mice and human donors. We confirmed that oxidative and hypoxic damage and estrogen deprivation can induce IL-6 and IL-8 export by human female, premenopausal stroma. We adapted a breast cancer cell co-culture model with injured stroma to determine the effect on stromal capacity to support dormancy. We adapted an osteoclast activation model to determine the capacity of injured stroma to activate osteoclasts. We also adapted a preosteoblast co-culture model to determine the role of osteoblasts in the micrometastatic cell niche responsible for recurrence of the dormant breast cancer. These experiments will serve to identify the adhesion and signaling necessary to maintain survival and resistance of dormant cancer cells.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2011
Accession Number
ADA550871

Entities

People

  • Robert Wieder

Organizations

  • University of Medicine and Dentistry of New Jersey

Tags

DTIC Thesaurus Topics

  • Bone Marrow
  • Bones
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Culture Media
  • Culture Techniques
  • Deprivation
  • Estrogens
  • Macrophages
  • Neoplasms
  • Osteoblasts
  • Oxidative Stress
  • Stem Cells
  • Stromal Cells
  • Survival
  • Tumor Cell Line

Readers

  • Immunology and Pathology
  • Oncology (Cancer Research).