Feasibility of a DNA-Based Combinatorial Array Recognition Surface (CARS) in a Polyacrylamide Gel Matrix
Abstract
We report initial attempts al developing a self-assembled combinatorial DNA biosensor array which may be capable of binding and identilYing vir1ually any soluble analyte that binds the array by pattern recognition, in effect making it a universal biosensor surface. Data are presented for differential binding pattcrns of various analytcs to one-dimensional arrays of combinatorial DNA concatamer libraries, which are spatially separatcd according to size and charge by electrophoresis in polyacrylamide gels. These DNA concatamer librarics arc essentially composed of single-stranded (ss) random DNA 60mers, which fonn a "'smear" pattern in gels following elcctrophoresis. Whcn used to bind and detect various analytcs or mixtures of analytes in the gel, we refer to the DNA smear as a "Combinatorial Array Recognition Surface" (CARS). Differences in intrinsic fluorescence scanning patterns of CARS gel strips were compared before and after addition of various analytes to the arrays to detcct binding pallems. Scans revealed a high level of reproducibility for individual CARS arrays in a given gel with or without bound analytes. Scan patterns between diffcrent CARS gel strips were initially less reproducible, but purification of the DNA library using spin columns prior to electrophoresis improved gel-to-gel reproducibility.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 12, 2007
- Accession Number
- ADA551688
Entities
People
- John G Bruno
- John L. Alls
- Johnathan L. Kiel
Organizations
- Air Force Research Laboratory