Human Neural Cell-Based Biosensor
Abstract
Human neural progenitors have a strong potential for use as cell-based biosensors for environmental toxins. Here, we report the following accomplishments in developing a neural cell-based biosensor. (1) We developed iPSC equivalents for the ESC-derived hNP1 neural progenitor and hN2 mixed neuronal cell lines, giving us the capability to create disease-specific neural cells from patient fibroblasts. (2) We continue to optimize our novel approach for hNP1 differentiation into dopaminergic neurons to increase yield beyond 50%, allowing us to create a more physiologically relevant, HTS-ready in vitro model for Parkinson s disease research. (3) We have begun pilot studies to differentiate hNP1 progenitors into motor neurons and astrocytes; these cell types have potential for being physiologically relevant in vitro models for botulinum toxin detection and neuron-glia interactions, respectively. (4) We developed HTS- and HCI-amenable assays for cell migration, cellular ATP, and neurite outgrowth. These assays have the potential as single or multiplexed assays to identify compounds with effects on neurogenesis (proliferation and differentiation) and to delineate mechanism-of-action for unknown neurotoxicants. (5) We developed an immunoblot based method for detecting botulinum toxin using the mixed neuronal hN2 cell line, thus creating a first generation human cellular model for botulinum toxin detection standard of comparison for existing and future models.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 11, 2011
- Accession Number
- ADA552021
Entities
People
- Allan Powe
- Jamie Chilton
- Steven L. Stice