Protection Against Dengue Virus by Non-Replicating and Live Attenuated Vaccines Used Together in a Prime Boost Vaccination Strategy

Abstract

A new vaccination strategy for dengue virus (DENV) was evaluated in rhesus macaques by priming with tetravalent purified inactivated virus (TPIV) or tetravalent plasmid DNA vaccines expressing the structural prME gene region (TDNA) then boosting 2 months later with a tetravalent live attenuated virus (TLAV) vaccine. Both vaccine combinations elicited virus neutralizing (N) antibodies. The TPIV/TLAV combination afforded complete protection against DENV 3 challenge at month 8. In a second experiment, priming with TPIV elicited N antibodies against all four serotypes (GMT 1:28 to 1:43). Boosting with TLAV led to an increase in the GMT for each serotype (1:500 to 1:1200 for DENVs 1, 3, and 4, and greater than 1:6000 for DENV 2), which declined by month 8 (GMT 1:62 for DENV 3, 1:154 for DENV 1, 1:174 for DENV 4, and 1:767 for DENV 2). After challenge with each one of the four DENV serotypes, vaccinated animals exhibited no viremia but showed anamnestic antibody responses to the challenge viruses.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2010
Accession Number
ADA552036

Entities

People

  • Julia Lynch
  • Monika Simmons
  • Robert Putnak
  • Timothy Burgess

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Animals
  • Blood
  • Cells
  • Clinical Trials
  • Dengue
  • Immunity
  • Immunization
  • Immunogenicity
  • Infection
  • Medical Personnel
  • Proteins
  • Rhesus Monkeys
  • Vaccination
  • Vaccines
  • Virus Diseases
  • Viruses
  • Yellow Fever

Fields of Study

  • Biology

Readers

  • Immunology
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology