Investigating the Role of Fatty Acid Metabolism and DecR1 Expression ErbB2-Driven Mammary Tumorigenesis and Metastasis Using a Transgenic Mouse Model Approach

Abstract

Cancer cell metabolism has become an increasingly active area of research in recent years, elucidating the importance of Warburg metabolism and its role in tumorigenesis. Fatty acid oxidation is diminished in favor of synthesis during the tumorigenic process. DecR1 is a fatty acid oxidation protein that is frequently downregulated in models of breast cancer. Using a mouse model approach, expression of DecR1 in mammary epithelial cells results in a delay in ductal outgrowth, part of normal mammary gland development. Additionally, when expression of DecR1 is driven in an ErbB2 mouse model of breast cancer, it confers a slight delay in tumor onset. However, expression is lost in the end-stage tumors, suggesting induction of fstty acid oxidation is selected against during tumorigenesis. This is further supported by the finding that na ve, uninduced tumor cells retain the capacity to undergo induction. Finally, orthotopic injection of na ve tumor cells into nude mice shows induction of DecR1 expression has a growth-suppressive effect. Taken together, this suggests fatty acid oxidation is antagonistic to tumorigenesis, however the long term impact of DecR1 expression is still not known

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2011
Accession Number
ADA552058

Entities

People

  • Jason Turpin

Organizations

  • McGill University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Chemical Compounds
  • Diseases And Disorders
  • Epithelial Cells
  • Fatty Acids
  • Gene Expression
  • Glands
  • Histological Techniques
  • Mammary Glands
  • Metabolism
  • Metabolomics
  • Neoplasms
  • Oxidation

Fields of Study

  • Biology

Readers

  • Aquatic Ecology
  • Molecular and Cellular Biology
  • Oncology (Cancer Research).