Use of Synthetic Antibodies Targeted to the Jak/Stat Pathway in Breast Cancer

Abstract

This proposal seeks to generate sABs (synthetic antigen binders) against the PRLr (prolactin receptor)signaling complex to systematically inhibit and modulate its important activities in breast cancer. This complex has clearly been demonstrated to play a significant role in development and spread of this disease, and yet the generation of pharmacologic agents that can specifically block their function has been slow. We hypothesize that sABs can be generated to the PRLr, CypA, and CypB proteins and delivered into the cell, where these reagents will block breast cancer growth, survival and spread. We have successfully generated sABs to CypA and B and the extracellular domain of PRLr and have begun their characterization. It is the intent of this proposal to validate the efficacy of these highly innovative reagents on a panel of breast cancer cells in vitro, with future translation into pre-clinical testing.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2010
Accession Number
ADA552192

Entities

People

  • Anthony A. Kossiakoff

Organizations

  • University of Chicago

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Bioassay
  • Biochemistry
  • Biology
  • Breast Cancer
  • Cells
  • Chemical Compounds
  • Chemistry
  • Computational Biology
  • Crystal Structure
  • Molecular Biology
  • Neoplasms
  • Proteins
  • Surface Plasmon Resonance
  • Surface Plasmons
  • Systems Biology
  • X Rays

Fields of Study

  • Biology
  • Medicine

Readers

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  • Molecular Biology and Genetics