Discrimination of Major Capsular Types of Campylobacter jejuni by Multiplex PCR
Abstract
The polysaccharide capsule (CPS) of Campylobacler jejuni is the major serodeterminant of the Pennel' serotyping scheme. There are 47 Penner serotypes of C. jejuni, 22 of which fall into complexes of related se.-otypes. A multiplex J CR method for determination of capsule types of Campy/obacler jejuni which is simpler and more affordable than classical Penner typing was developed. Primers specific for each capsule type were designed on the basis or a database of gene setJuences from the variable capsule loci of 8 strains of major serotypes sequenced in this study and 10 published sequences of other serotypes. DNA sequence analysis revealed a mosaic nature of the capsule loci, suggesting reassnrtment of genes by horizontal transfer, and demonstrated a high degree ot' conservation of genes within Penner complexes. The multiplex PCR can distinguish 17 individual serotypes in two PCRs with sensitivities and specificities ranging from 90 tn 100% using 244 strains of knnwn Penner type.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2011
- Accession Number
- ADA552215
Entities
People
- Carl Mason
- Cory N. Cates
- Frederic Poly
- Jennifer Ju
- Marc Schulman
- Margaret Kanipes
- Oralak Serichatalergs
- Patricia Guerry
Organizations
- Naval Medical Research Center