Amylase and Lipase Detection in Hemorrhaged Animals Treated with HBOC-201

Abstract

HBOC-201 may alter lipase and amylase detection on chemistry analyzers using optical methods and affect pancreatic function after trauma. Amylase and lipase measurements were correlated against HBOC-201 to evaluate interference on samples spiked with 0-6g/dL HBOC-201. The detection threshold was 2.5g/dL or none when measured, respectively, on Vitros 250 or Advia 1650 instruments. Amylase and lipase from blood samples collected from 55% EBV hemorrhaged Yucatan min-pigs showed peaks around 24-48 hours. Amylase increase was not significant between treatments but lipase was higher in HBOC-201-treated animals. Animals particularly affected by the injury had elevated enzymes after hemorrhagic shock, without significant clinical consequences.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2011
Accession Number
ADA552324

Entities

People

  • Daniel Freilich
  • Françoise Arnaud
  • Richard Mccarron

Organizations

  • Naval Medical Research Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Absorption
  • Albumins
  • Analyzers
  • Biomedical Research
  • Blood
  • Blood Transfusions
  • Cardiovascular Physiological Phenomena
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Health Services
  • Hemoglobin
  • Hemorrhage
  • Hemorrhagic Shock
  • Laboratory Animals
  • Measurement
  • Test Methods

Readers

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