Targeting Breast Cancer with a Steroid Adapter to Inhibit DNA Repair

Abstract

We have developed an electrophoretic mobility shift assay for assembly of the core ligase complex involved in nonhomologous end joining (NHEJ). The assay can detect inhibition of complex formation upon linkage to estrogen receptor. We have demonstrated that Cernunnos is an excellent target for disruption of NHEJ. We have shown that B. subtilis Sfp enzyme can attach biotin-CoA and TAMRA-CoA conjugates to ybbR-tagged Cernunnos without disrupting its ability to stimulate end joining. We synthesized hydroxytamoxifen-CoA as a prototype adapter for attachment to ybbR-tagged Cernunnos. This will allow us to demonstrate that end joining is preserved in the absence of estrogen receptor, and disrupted in its presence.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2011
Accession Number
ADA552373

Entities

People

  • Gilbert Chu

Organizations

  • Stanford University

Tags

DTIC Thesaurus Topics

  • Adapters
  • Amino Acids
  • Assembly
  • Attachment
  • Breast Cancer
  • Chemical Synthesis
  • Chemistry
  • Estrogens
  • Inhibition
  • Ionizing Radiation
  • Joining
  • Mass Spectrometry
  • Mass Spectroscopy
  • Molecules
  • Prototypes
  • Small Molecules
  • Spectroscopy

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Electrical Engineering
  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology