Epigenetic Control of Prolyl and Asparaginyl Hydroxylases in Prostate Cancer

Abstract

In many solid tumors, including prostate cancer, hypoxia-inducible factors (HIF) are up-regulated compared to their normal tissue counterparts. These HIF molecules are transcription factors, and up-regulate metabolic and angiogenic proteins in the cancer. Recently, a set of proteins known as prolyl and asparaginyl hydroxylases (PHD and AHD respectively) have been shown to be essential in the regulation of HIF, and in some cancers have been transcriptionally and translationally silenced. We therefore proposed a study that focuses on the epigenetic control of these crucial enzymes. In this report, we present data demonstrating our first findings of PHD expression in prostate cancer cell lines as well as expanding our studies to relevant human samples. Furthermore, we begin to identify specific epigenetic mechanisms that may play a major role in the transcriptional and translational control of these enzymes. Last, we will explain our future direction of the project after the award period has expired.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2011
Accession Number
ADA552430

Entities

People

  • Adam Case

Organizations

  • University of Iowa

Tags

DTIC Thesaurus Topics

  • Adaptive Immunity
  • Blood
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Data Analysis
  • Environmental Health
  • Epithelial Cells
  • Free Radicals
  • Gene Expression
  • Genetics
  • Lymphocytes
  • Neoplasms
  • Peptides
  • Proteins
  • Regression Analysis

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics