Francisella tularensis Molecular Typing Using Differential Insertion Sequence Amplification
Abstract
Tularemia is a potentially fatal disease that is caused by the highly infectious and zoonotic pathogen Francisella tularensis. Despite the monomorphic nature of sequenced F. tularensis genomes, there is a significant degree of plasticity in the organization of genetic elements. The observed variability in these genomes is due primarily to the transposition of direct repeats and insertion sequence (IS) elements. Since current methods used to genotype F. tularensis are time-consuming and require extensive laboratory resources, IS elements were investigated as a means to subtype this organism.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2011
- Accession Number
- ADA552443
Entities
People
- Amanda M. Bartling
- Marilynn A. Larson
- Michael P. Dempsey
- Paul D. Fey
- Peter C. Iwen
- Stephen C. Francesconi
- Steven H. Hinrichs
Organizations
- Naval Medical Research Center