Genome-Wide Profiling of Humoral Immune Response to Coxiella burnetii Infection by Protein Microarray
Abstract
Comprehensive evaluation of the humoral immune response to Coxiella burnetii may identify highly needed diagnostic antigens and potential subunit vaccine candidates. Here we report the construction of a protein microarray containing 1901 C. burnetii ORFs (84% of the entire proteome). This array was probed with Q-fever patient sera and naive controls in order to discover C. burnetii-specific seroreactive antigens. Among the 21 seroreactive antigens identified, 13 were significantly more reactive in Q-fever cases than naive controls. The remaining eight antigens were cross-reactive in both C. burnetii infected and naive patient sera. An additional 64 antigens displayed variable seroreactivity in Q-fever patients, and underscore the diversity of the humoral immune response to C. burnetii. Nine of the differentially reactive antigens were validated on an alternative immunostrip platform, demonstrating proof-of-concept development of a consistent, safe, and inexpensive diagnostic assay alternative. Furthermore, we report here the identification of several new diagnostic antigens and potential subunit vaccine candidates for the highly infectious category B alphaproteobacteria, C. burnetii.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2010
- Accession Number
- ADA552695
Entities
People
- Adam Vigil
- Chien-Chung Chao
- Douglas M. Molina
- Hua-wei Chen
- Jozelyn Pablo
- Philip L. Felgner
- Rie Nakajima-sasaki
- Rocio Ortega
- Wei-mei Ching
Organizations
- Naval Medical Research Center