Cloning and Expressing Recombinant Protective Antigen Domains of B. anthracis
Abstract
Protective antigen (PA) of Bacillus anthracis is a four-domain protein that is required for transport of edema factor (EF) and lethal factor (LF) into host cells during anthrax infection. Each of the four domains of PA plays a role in infection: host cell receptor binding, domain 4, PA heptamerization and host membrane insertion, domains 2 and 3, and EF and LF recognition and intracellular transport, domain 1. This report details our efforts to produce single- and multi-domain combinations of the PA protein. All multi-domains (1-2, 2-3, 3-4) and three of the four single domains (1, 2, 4) have been cloned, expressed, and purified and will be used for future efforts in domain mapping of PA-binding antibodies and peptide reagents.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2011
- Accession Number
- ADA553045
Entities
People
- Deborah A. Sarkes
- Dimitra N Stratis-Cullum
- Irene Val-addo
- Joshua M. Kogot
- Paul M. Pellegrino
Organizations
- United States Army Research Laboratory