Forensic Analysis of Human DNA from Samples Contamined with Bioweapons Agents

Abstract

The purpose of this research project was to further characterize and improve a preliminary protocol for removal of bacterial agents and toxins from samples that would undergo DNA analysis. The protocol must result in samples that are free of infectious or toxic material, but still generate DNA of adequate quantity and quality to meet RCMP standards for identification based on DNA. In order to obtain and identify possible criminals from DNA evidence, human samples must undergo various steps to isolate and analyse the DNA in a forensic laboratory. These steps consist of (1) the isolation of DNA from samples collected at the crime scene, (2) quantification of the DNA (3) amplification of specific regions on the human chromosomes, (4) analysis of the sequence of amplified DNA, and (5) comparison with possible suspects whose DNA has been banked in the National DNA Data Bank (NDDB) or whose DNA has been collected as part of the investigation.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2011
Accession Number
ADA553107

Entities

People

  • Jason Timbers
  • Kathryn Wright

Organizations

  • Royal Canadian Mounted Police

Tags

DTIC Thesaurus Topics

  • Alcohols
  • Bacteria
  • Biological Factors
  • Biological Toxins
  • Capillary Electrophoresis
  • Cells
  • Chemistry
  • Chromosomes
  • Crime
  • Genetics
  • Heat Treatment
  • Identification
  • Materials
  • Medical Personnel
  • Microbiology
  • Microorganisms
  • Spores

Readers

  • Environmental Engineering.
  • Molecular Genetics