Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run

Abstract

Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this technique outside of genome finishing. We have demonstrated the production of multiple bacterial maps using a single set of consumables; this significantly reduces the time and expense of map production.

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Document Details

Document Type
Technical Report
Publication Date
Nov 21, 2011
Accession Number
ADA553564

Entities

People

  • Benjamin C. Kirkup Jr.
  • Emil Lesho
  • James E. Lee
  • Matthew C. Riley

Organizations

  • Walter Reed Army Institute of Research

Tags

DTIC Thesaurus Topics

  • Assembly
  • Bacteria
  • Chromosomes
  • Department Of Defense
  • Electronic Mail
  • Environment
  • Escherichia Coli
  • Gel Electrophoresis
  • Information Operations
  • Low Resolution
  • Molecules
  • Phosphodiesterases
  • Production
  • Quality Control
  • Sequences
  • Throughput
  • United States

Fields of Study

  • Biology

Readers

  • Computational Modeling and Simulation
  • Geodesy
  • Molecular Genetics