An Evaluation of the PCB-TOX-SPOT Water Toxicity Test

Abstract

The United States Army Center for Environmental Health Research (USACEHR) is developing an Environmental Sentinel Biomonitor (ESB) system to test Army drinking water supplies for the presence of toxic industrial chemicals (TICs). One of the technologies considered for inclusion in the ESB system is the PCB TOX-SPOT Chemiluminescence Test, a rapid assay that measures changes in luminescence of the bacteria Photobacterium leiognathi as an indicator of toxicity. The TOX-SPOT test was able to respond to only 5 of 18 chemicals in a test set identified by an Army user group within a desired sensitivity range. Further, the TOX-SPOT kit has three reagents that must be stored at -14?C, which is undesirable for field use. Evaluation of TOX-SPOT reagents held under refrigeration at 6?C during a 12 month storage period produced inconsistent toxicity test results. Based on these test results, the TOX-SPOT test is not recommended for inclusion as part of the ESB system.

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Document Details

Document Type
Technical Report
Publication Date
Sep 15, 2011
Accession Number
ADA553637

Entities

People

  • David E. Trader
  • William Van Der Schalie

Organizations

  • U.S. Army Center for Environmental Health Research

Tags

DTIC Thesaurus Topics

  • Chemical Synthesis
  • Chemistry
  • Detection
  • Detectors
  • Drinking Water
  • Environmental Health
  • Liquid Chromatography
  • Luminescence
  • Sodium Compounds
  • Spot Tests
  • Statistical Analysis
  • Test And Evaluation
  • Test Methods
  • Toxicity
  • United States
  • Warning Systems
  • Water Supplies

Readers

  • Environmental Engineering.
  • Regression Analysis.