Identification of Druggable Proteins Regulating Receptor Recycling in Breast Cancer Cells
Abstract
Increased receptor recycling leads to elevated receptors on the plasma membrane, thereby augments signaling cascades important in cancer progression and drives tumor aggressiveness. Inhibition of the altered recycling pathway in breast cancer cells would thus dampen oncogenic signaling and increase the efficacy of therapy. We propose to monitor EGFR trafficking using a newly developed AP-tag (or Avitag) system. We have generated functional constructs for the label of EGFR using AP-tag and established the labeling technique in the lab. Furthermore, we also generated several stable cell lines expressing the AP-EGFR.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2011
- Accession Number
- ADA554389
Entities
People
- Guangwei Du
- Melissa Wilmarth
- Ping Wu
- Yoshiya Yonekubo
Organizations
- University of Texas Health Science Center at Houston