DCIS-Specific MicroRNA in Cancer Stem Cell

Abstract

More than 20% of breast cancer patients detected by mammography are DCIS and this number keeps on increasing (1). Although DCIS is a non-invasive benign tumor, it is considered a precursor of malignant cancer. Therefore, understanding the molecular changes from normal cell to DCIS is of paramount importance and it is under intensive study. However, the exact mechanism of normal-DCIS transition is still not well understood. The purpose of this project is to identify specific microRNAs in tumor stem cells that are responsible for the formation of DCIS. The results of our microRNA array analysis for CSCs from normal and DCIS cells revealed that the expression of a series of microRNA are changed during the transition from normal to DCIS in CSCs. Particularly, we found that miR29a and miR29c function as suppressors of DCIS by blocking the expression of SREBP1, suggesting that modulation of these microRNAs in CSCs triggers the initiation of DCIS. Because the major target of miR29a and miR29c is SREBP, it is possible that this gene can be a potential candidate as a preventive drug for DCIS. Alternatively, the expression of miR29a and miR29C can be restored by a small molecule. It would be ideal if this can be accomplished with specific nutrition. However, this requires further investigation.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2011
Accession Number
ADA554452

Entities

People

  • Kounosuke Watabe

Organizations

  • Southern Illinois University

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Alkanes
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Carcinoma
  • Cell Line
  • Cells
  • Department Of Defense
  • Magnetic Separation
  • Microarray Analysis
  • Molecules
  • Neoplasms
  • Pcr Testing
  • Small Molecules
  • Stem Cells
  • Transitions

Fields of Study

  • Biology

Readers

  • Military/Explosive Ordnance Disposal (EOD) Technology
  • Molecular Biology and Genetics
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology