Targeting the I-Kappa-B Kinase Epsilon (IKKe) for Breast Cancer Therapy
Abstract
IKK(Epsilon) has been recently identified as a breast cancer oncogene. Although it is believed that the kinase activity of IKK(epsilon) is essential for its oncogene activity, its mechanism in turmogenesis and therapeutic potential are not fully understood. In this study, we employed synthetic siRNAs to silence IKK(epsilon) in breast cancer cells. Eight siRNA were designed and two of them showed significant silencing effect up to 78%. Various biologic activity studies in breast cancer cells were examined using these two potent IKK(epsilon) siRNAs. Silencing of IKK(epsilon) using siRNA resulted in decreases in clonogenicity, migration, invasion, and cell proliferation. The anti-proliferation effect of IKK(epsilon) siRNA was mediated by arresting cells in G0/G1 phase. Moreover, we demonstrated that the silencing of IKK(epsilon) inhibited the NF- B basal activity as well as its downstream genes. The present study provided the first evidence that silencing IKK(epsilon) using synthetic siRNA inhibited the invasiveness properties and proliferation of breast cancer cells. Our results suggested that silencing the oncogene IKK(epsilon) using synthetic siRNA may offer a novel therapeutic strategy for breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2009
- Accession Number
- ADA554623
Entities
People
- Bin Qin
- Kun Cheng
Organizations
- Kansas City University