Systemic And Local Vaccination Against Breast Cancer With Minimum Autoimmune Sequelae
Abstract
Our goal is to eliminate the tumor by vaccination and local ablation to render long-term immune protection without excessive autoimmune sequelae. Complimenting this regimen is systemic modulation of natural/induced Treg (iTreg) and intratumoral expression of immune augumenting cytokines. The two aims are to (1) Test the hypothesis that cryosurgery of cytokine enriched tumors amplifies Her-2 vaccine response, and (2) Test the hypothesis that disabling iTreg conversion enhances Her-2 immunity, not autoimmunity. In aim 1, we will measure immune response to tumor associated Her-2 by cryotherapy with and without DNA vaccination and the effect of intratumoral expression of cytokine. In aim 2, we will evaluate the degree of iTreg conversion in tumor bearing mice, measure Her-2 vaccine response in Her-2+TIEG1-/- mice which do not generate iTreg, and measure the induction of experimental autoimmune thyroiditis in iTreg deficient mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2011
- Accession Number
- ADA555055
Entities
People
- Wei-Zen Wei
Organizations
- Wayne State University