Laser Capture Microdissection Assisted Identification of Epithelial MicroRNA Expression Signatures for Prognosis of Stage I NSCLC

Abstract

The primary aim of this project is to laser microdissect stage I lung cancer samples, perform microRNA profiling of the epithelial and stromal components and develop component specific signatures of prognosis. This report summarizes the work performed on the project so far. A significant body of work has been performed to optimize the extraction of RNA from formalin fixed paraffin embedded lung cancer tissue and a working protocol established. These finding are useful to the scientific community and have been submitted for publication. Fifty seven of the proposed 77 stage I lung cancer samples have been microdissected this far into their epithelial and stromal component separated. Enough dissection has been performed to successfully acquire 400 ng of total RNA from each component.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2011
Accession Number
ADA555298

Entities

People

  • Saikrishna Yendamuri

Organizations

  • Health Research, Incorporated

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alkanes
  • Biomedical Research
  • Cancer
  • Ceramic Materials
  • Data Analysis
  • Extraction
  • Gene Expression
  • Health Services
  • High Resolution
  • Information Science
  • Least Squares Method
  • Lung Cancer
  • Neoplasms
  • Nucleic Acids
  • Silicon Carbide
  • Statistical Analysis
  • United States

Fields of Study

  • Biology

Readers

  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Directed Energy