Preclinical Studies of Induced Pluripotent Stem Cell-Derived Astrocyte Transplantation in ALS

Abstract

We have made substantial progress in obtaining skin biopsies with subsequent fibroblast cultures of a number of sporadic ALS, familial ALS, and control subjects in the last year. We have collected a variety of ALS phenotypes including slow progressing ALS, primary lateral sclerosis (PLS), lower motor neuron only ALS (LMN), ALS with frontotemporal dementia (ALS/FTD) and appropriate controls (Total of approximately 85 subjects collected to date). In addition, we have also obtained 28 samples from subjects with familial ALS (these include SOD1, ANG, FIG4, and FUS mutations). In collaboration with Johns Hopkins Investigators, we have developed 22 FALS iPSC lines, 8 sporadic ALS iPSC lines and 4 control iPSC lines. We have been using a method to allow for long-term differentiation of iPS cells into astroglia.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2011
Accession Number
ADA555307

Entities

People

  • Hongjun Song
  • Nicholas J Maragakis

Organizations

  • Johns Hopkins University

Tags

DTIC Thesaurus Topics

  • Animal Structures
  • Astrocytes
  • Biomedical Research
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Dementia
  • Diseases And Disorders
  • Fibroblasts
  • Motor Neurons
  • Mutations
  • Neurons
  • Phenotypes
  • Precursors
  • Sclerosis
  • Stem Cells
  • Teamwork

Readers

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Technology Areas

  • Biotechnology