Regulation of Mammary Tumor Formation and Lipid Biosynthesis by Spot14

Abstract

The objective of this study is to develop models to causatively link Spot 14 (S14) expression in the mammary epithelium and tumor formation, metabolism, and metastasis. Our hypothesis is that S14 promotes de novo lipid biosynthesis in tumor cells, causing enhanced proliferation and ultimately, tumor metastasis. Using a genetic model of spontaneous tumorigenesis, we have found that the presence of S14 significantly accelerates tumor latency, and is associated with branching and budding of the non tumor- bearing mammary glands. Additionally, we have established cell lines from S14-/- mammary epithelium, transformed with the Polyoma Middle T antigen (PyMT) or Neu oncogenes. These lines will be used in xenotransplant experiments to test the requirement of S14 for mammary tumor growth and cellular lipid metabolism.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2011
Accession Number
ADA555801

Entities

People

  • Elizabeth Wellberg

Organizations

  • University of Colorado Boulder

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Line
  • Cells
  • Department Of Defense
  • Diseases And Disorders
  • Dna Microarrays
  • Epithelial Cells
  • Fatty Acids
  • Lipid Metabolism
  • Mammary Glands
  • Mass Spectrometry
  • Medical Personnel
  • Metabolic Pathways
  • Metabolism
  • Metabolomics
  • Microarray Analysis
  • Neoplasms

Readers

  • Molecular Biology and Genetics
  • Prostate Cancer Biology.

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech