Assessments of Tumor Extracellular pH with PARACEST MRI

Abstract

In this research project an approach to measure pH by Chemical Exchange Saturation Transfer (CEST) MRI is developed. We describe the characterization of a PARACEST MRI contrast agent, Yb-DO3A-oAA, for pH measurement. The pH measurement is independent of concentration and T1sat relaxation times, and covers a wider pH measurement range than alternative methods. We also developed a new method for fitting PARACEST. To simplify MRI acquisition of CEST spectra a new MRI method was developed, arrayed CEST-FISP. The parameters of this method were optimized for use with PARACEST agents. With this techniques established we translated Yb-DO3A-oAA from in vitro to in vivo use to measure pH in a MDA-MB-231 tumor model and mouse muscle. The pharmacodynamics of Yb-DO3A-oAA were investigated and the fitting algorithm was extended to in vivo tissues.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2012
Accession Number
ADA559236

Entities

People

  • Vipul R. Sheth

Organizations

  • Case Western Reserve University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acquisition
  • Algorithms
  • Biochemistry
  • Biomedical Engineering
  • Biomedical Research
  • Chemical Shifts
  • Chemical Synthesis
  • Chemistry
  • Magnetic Fields
  • Magnetic Resonance
  • Magnetic Resonance Imaging
  • Mass Spectrometry
  • Measurement
  • Neuroimaging
  • Relaxation Time
  • Spectra
  • Spectroscopy

Readers

  • Calculus or Mathematical Analysis
  • Environmental Engineering
  • Medical Imaging.