Targeting Breast Cancer with a Steroid Adapter to Inhibit DNA Repair
Abstract
We have developed an efficient assay capable of detecting assembly of the core ligase complex involved in nonhomologous end joining (NHEJ) of DNA double-strand breaks. The assay can also detect inhibition of complex formation upon linkage to a large protein such as estrogen receptor. We have shown that Cernunnos is an ideal target for disrupting NHEJ. Using recombinant proteins, we have found regions in Cernunnos that can be targeted for disrupting repair only in cells expressing estrogen receptor. Finally, we have performed experiments to demonstrate the feasibility of each individual step in the chemical synthesis of the adapter.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2010
- Accession Number
- ADA559937
Entities
People
- Gilbert Chu
Organizations
- Stanford University