Proteomic Analysis of Prostate Cancer Field Effect
Abstract
We have undertaken a novel proteomic approach to identify proteins altered in both cancer and benign cells near the cancers. This is to take advantage of the field effect of the cancer, the malignancy associated changes in normal cells, to discover cancer altered protein that could be more abundant in serum or urine since they would come from both benign and cancer cells. We have started statistical evaluation of proteins comparing proteins in cancer (CaP), benign near (BN), benign distant (BD), and benign prostatitic hyperplasia (BPH) tissue areas of 7 prostates. In the evaluation of proteins found in 5 of at least 7 prostate specimens, 3 were changed in levels of expression comparing CaP vs BN (calreticulin, peptidylprolyl isomerase B, transferin), 4 comparing CaP vs BD (beta-microseminoprotein, filamin A, histone cluster 2, four and half LIM domains 1). However, 29 proteins were significantly varied in expression in CaP vs BPH comparisons. Calculations using missing value imputation is now being carried out to seek possible other biomarker candidate proteins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2011
- Accession Number
- ADA560531
Entities
People
- Michael J. Wilson
Organizations
- University of Minnesota