Validation of Biomarkers Predictive of Recurrence Following Prostatectomy
Abstract
To validate biomarkers of recurrence following radical prostatectomy. Scope: Perform genomic profiling of test and validation sets from formalin-fixed samples with longterm follow up data. Major Findings: We have published a manuscript in The American Journal of Pathology describing our set of biomarkers, and presented these data orally at the American Society of Investigative Pathology annual meeting in Washington, DC on April 11, 2011. We have optimized our method for RNA-seq analysis and collected 171 patient samples, of which 108 have passed RNA QC. We are in the process of preparing RNA-seq libraries and anticipate generating large datasets for analysis in the next few months. We have applied our published biomarkers to publicly available datasets and shown that they are predictive. We have demonstrated that four protein biomarkers (BID, SIM2s, FBP1, and RAD23B) are significantly associated with recurrence in prostate cancer. These immunohistochemistry assays may be useful for predicting recurrence in clinical cases.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2012
- Accession Number
- ADA560790
Entities
People
- Carlos S Moreno
Organizations
- Emory University