Analysis of Dachsous2 in Breast Cancer Progression and Recurrence

Abstract

Microarray analysis of tumors women with axillary node negative (ANN) breast cancer revealed that a cDNA for Dachsous 2 (Ds2) is one of the most highly discriminatory genes in tumors from patients who had experienced early recurrence, compared to tumors from patients who did not recur for at least ten years (p= 1.1 x 10- 5 by students t-test). This suggests that increased transcription of Ds2 may be a predictive indicator of recurrence in ANN breast cancer. Ds2 encodes a large cell adhesion molecule implicated in planar polarity and cell proliferation, To determine the significance of the increased levels of Ds2 in recurrence we will: 1) Validate gene expression array results and determine if Ds2 proteins levels increase in tumors 2) Determine if PCP or Hippo pathway gene expression is altered upon Ds2 overexpression. 3) Determine the effects of altering Ds2 levels on proliferation and tumor susceptibility in the mouse.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2011
Accession Number
ADA561999

Entities

People

  • Helen Mcneill
  • Ian Hester
  • Irene Andrulis
  • Kristine Louis
  • Yonit Tsatskis

Organizations

  • Mount Sinai Hospital, Toronto

Tags

DTIC Thesaurus Topics

  • Adhesion
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cell Polarity
  • Cells
  • Gene Expression
  • Genetic Structures
  • Genetics
  • Health Services
  • Medical Personnel
  • Microarray Analysis
  • Molecules
  • Neoplasms
  • Polarity
  • Polymerase Chain Reaction

Readers

  • Data Mining and Knowledge Discovery.
  • Molecular Genetics
  • Oncology (Cancer Research).