Desferrioxamine for Stimulation of Fracture Healing and Revascularization in a Bone Defect Model

Abstract

Our study explored local delivery of deferroxamine with and without bone allograft as a means to accelerate fracture healing in a tibia defect model of the rat. Experimental groups in the first study included: control, CS: calcium sulfate annulus around spacer at defect, CS/DBA: CS implant and demineralized bone allograft (DBA); CS/DFO: DFO (300 g) loaded CS implant, CS/DFO/DBA: DFO loaded CS implant with DBA allograft. Experimental groups in the second study included: control, DBA, LDFO/ DBA: DBA soaked with DFO (10 g), H-DFO/DBA: DBA soaked with DFO (100ug). Fractures were evaluated at 6 weeks postinjury for radiographic cortical bridging, CT mineralized callus volume and torsional properties. The bulk CS annulus was found to impair healing compared to the control in the 1st study. In the 2nd study L-DFO/DBA was found to increase cortical bridging and torsional strength relative to the control. Bulk form CS is not an effective method of delivery of DFO to stimulate fracture healing. Low dosage DFO treatment directly to DBA was found to enhance the ability of DBM to stimulate fracture healing, while higher dosage DFO treatment of DBA was not effective.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2012
Accession Number
ADA562175

Entities

People

  • Laurence Dahners
  • Paul Weinhold

Organizations

  • University of North Carolina at Chapel Hill

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Allografts
  • Biomedical Engineering
  • Body Weight
  • Bone And Bones
  • Calcium
  • Calcium Compounds
  • Cells
  • Chelate Compounds
  • Endothelial Cells
  • Engineering
  • Growth Factors
  • Materials
  • Mechanical Properties
  • North Carolina
  • Peptides
  • Surgery
  • Wounds And Injuries

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