Response of Human Prostate Tissue to Hypofractionated Ionizing Radiation
Abstract
The aim of this proposal was to determine the differences in radiobiological response of human prostate tissue to conventional and hypofractionated radiotherapy. Specifically, this proposal characterized the predominant DNA damage response pathway from human prostatectomy specimens in response to a conventional or hypofractionated dose of ionizing radiation. We demonstrate that normal prostate tissue and prostate cancer can be cultured ex vivo using a dynamic culture system and used to study the radiobiology of human prostate tissue. Normal prostate tissue responds to ionizing radiation with predicted repair foci (gamma-H2AX and Rad51), and a marker for cellular stress, p53. The DNA damage response in normal glands appears to predominate in the basal cell layer. Prostate cancer epithelium responds to ionizing radiation with predicted early repair foci (gamma-H2AX), but in contrast to normal prostate epithelium, homologous repair foci (Rad51) were not demonstrated. The ex vivo organ culture technique will be optimized to mimic the indolent nature of prostate tissues and retain the integrity of the stroma. This system will promote testing of clinically relevant questions for translational prostate cancer research.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2012
- Accession Number
- ADA562901
Entities
People
- Thomas C. Sroka
Organizations
- University of Arizona