Construction of a Bacterial Cell that Contains Only the Set of Essential Genes Necessary to Impart Life

Abstract

We are working towards the goal of creating a bacterium that contains only the set of genes that are essential for life. Toward that end, we have conducted transposon mutagenesis experiments, designed to identify genes that are not needed for life under laboratory conditions. We have also removed several large elements from the starting genome (M. mycoides JCVI-syn1.0). The reduced genome was successfully transplanted into recipient cells and found to be viable and to grow with a reasonable doubling time. We have begun work on an approach to design and synthesize a minimal genome.

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Document Details

Document Type
Technical Report
Publication Date
Aug 16, 2012
Accession Number
ADA563068

Entities

People

  • Anthony Yee
  • Clyde Hutchison
  • Hamilton Smith
  • John I Glass

Organizations

  • J. Craig Venter Institute

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Assembly
  • Attachment
  • Construction
  • Contracts
  • Department Of Defense
  • Energy Management
  • Energy Production
  • Governments
  • Manufacturing
  • Micro-Machines
  • Molecules
  • Organizational Structure
  • Platforms
  • Production
  • Sequences
  • Viability

Fields of Study

  • Biology

Readers

  • Mathematics or Statistics
  • Molecular Genetics

Technology Areas

  • Biotechnology