Role of Protein Kinase C Epsilon in Prostate Cancer and Metastasis
Abstract
The altered balance in the expression of PKC isozymes is a distinguished feature of cancer. One of the most notable alterations in epithelial cancers is the upregulation of PKC . This kinase has emerged as a potential oncogene and tumor biomarker, however, little is known regarding a potential causality between its upregulation and cancer development. In this research we wished to understand the role played by PKC in prostate cancer cells and establishes the proof of principle of PKC inhibition as a putative therapeutic strategy. Using a RNAi approach we found that PKC inhibition decreases size of tumor generated by PC3-ML cells in athymic/balb-c mice. Accordingly, immunohistochemical analysis of xenografts 10 days after inoculation showed a marked induction of cell death in PKC depleted cells. In addition we found that this kinase may be relevant in attachment, anchorage-dependent and anchorage- independent growth of PC3-ML cells. Preliminary data showed that PKC depletion markedly impaired the ability of PC3 to migrate and PC3-ML to invade and migrate. In summary, our results argue for a role of PKC in prostate cancer development and metastasis, highlighting its potential as a therapeutic target.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2012
- Accession Number
- ADA563092
Entities
People
- Alvaro Gutierrez-uzquiza
Organizations
- University of Pennsylvania