Androgenic Regulation of White Adipose Tissue-Prostate Cancer Interactions
Abstract
Adipocyte stromal cells from WAT of both Glipr1 wild type adult males and Glipr1 knock out adult males were isolated and used for coculturing experiment. Coculturing prostate cancer cells (VCaP, RM-9, PC-3) with ASCs from WAT of Glipr1 KO and Glipr1 WT resulted in increased growth of the cancer cells compared to the cells grown without the ASCs. Moreover, the prostate cancer cells grown with Glipr1 KO ASCs resulted in higher cell growth rate compared to the cells grown with Glipr1 WT ASCs. Similar results were found after the prostate cancer cells were incubated with ASCs-CM from both Glipr1 KO and WT. The cells incubated with Glipr1 KO ASCs-CM resulted in higher cell growth number compared to the cells grown with Glipr1 WT ASCs-CM, indicating the presence of differential levels of factors that promote cell growth. When the ASCs-CM from Glipr1 WT and KO were analyzed and compared, we detected numerous factors that promote tumor cell growth and invasion. As expected, the levels of these factors that promote cancer cell growth and invasion were much higher in Glipr1 KO ASCs-CM than in Glipr1 WT ASCs-CM.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2012
- Accession Number
- ADA563849
Entities
People
- Timothy C Thompson
Organizations
- The University of Texas MD Anderson Cancer Center