Human Neural Cell-Based Biosensor

Abstract

The purpose of this project is to develop in vitro, cell based biosensors for environmental toxins. By using ArunA s neural cell lines derived from both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), our goal is to provide a human neural cell based biosensor that is a more biologically relevant model of human physiology. This report focused on the differentiation of hESC- and hiPSC-derived neural progenitor cells and describes progress in these major areas: (1) characterization of induced pluripotent stem cell derived neural progenitor cells, (2) directed differentiation of hESC- and hiPSC-derived progenitor cells into dopaminergic neurons, (3) directed differentiation of neural progenitor cells into astrocytes and (4) cell culture medium development for the maintenance and differentiation of ArunA s neural cell lines as sensor elements for neurotoxicity.

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Document Details

Document Type
Technical Report
Publication Date
Jul 05, 2012
Accession Number
ADA563923

Entities

People

  • Jamie Chilton
  • Steven L. Stice

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Astrocytes
  • Biosensors
  • Cell Line
  • Cells
  • Contracts
  • Culture Techniques
  • Department Of Defense
  • Electronic Mail
  • Instructions
  • Military Research
  • Neurons
  • Parkinson'S Disease
  • Physiology
  • Stem Cells

Fields of Study

  • Biology
  • Chemistry

Readers

  • Molecular and Cellular Biology

Technology Areas

  • Biotechnology