Induced Pluripotent Stem Cells as Potential Therapeutic Agents in NF1

Abstract

In this project, we are seeking to use induced pluripotent stem (iPS) cell technology as a potential therapy in NF1. In the first years, we have successfully produced iPS cells from fobroblasts from Nf1 +/-mice and characterized the properties of these cells, which include growth in clusters, expression of stem cell markers, normal karyotype, and the ability to form teratomas in mice. We also created a targeting "gene-repair" vector and have used this vector to to replace the defective Nf1 allele in these iPS cells. We then showed that we could differentiate iPS cells into Schwann-like cells in vitro, enabling drug sensitivity studies.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2012
Accession Number
ADA564162

Entities

People

  • Jonathan Chernoff

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Culture Techniques
  • Epithelial Cells
  • Medical Personnel
  • Neoplasms
  • Pcr Testing
  • Precursors
  • Sensitivity
  • Stem Cells
  • Targeting

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech