Human Neural Cell-Based Biosensor

Abstract

The purpose of this project is to develop in vitro, cell based biosensors for environmental toxins. By using ArunA s neural cell lines derived from both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), our goal is to provide a human neural cell based biosensor that is a more biologically relevant model of human physiology. This report describes the progress made in these major areas: (1) directed differentiation of hESC- and hiPSC-derived neural progenitor cells into dopaminergic neurons; (2) directed differentiation of hESC- and hiPSC-derived neural progenitor cells into astrocytes; (3) cell culture medium development for the maintenance and differentiation of ArunA s neural cell lines as sensor elements for neurotoxicity; and (4) label-free, adhesive signature-based microfluidic cell separation.

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Document Details

Document Type
Technical Report
Publication Date
Oct 31, 2012
Accession Number
ADA566123

Entities

People

  • Jamie Chilton
  • Steven L. Stice

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Adhesives
  • Astrocytes
  • Biosensors
  • Cell Line
  • Cells
  • Contracts
  • Culture Techniques
  • Department Of Defense
  • Electronic Mail
  • Gene Expression
  • Instructions
  • Military Research
  • Neurons
  • Physiology
  • Stem Cells

Fields of Study

  • Biology
  • Chemistry

Readers

  • Immunology and Pathology
  • Molecular and Cellular Biology
  • Neural Network Machine Learning.

Technology Areas

  • Biotechnology