Reparative Medicine: Production of Erythrocytes & Platelets from Human Embryonic Stem Cells

Abstract

This project was focused on production of megakaryocytes and platelets from human pluripotent stem cells - originally human embryonic stem cells (hESC), but subsequently, as the technology became available, from human induced pluripotent stem cells (hiPSC). We developed a serum-free system, and also created our own cell-free extracellular matrix extracts instead of live feeder cells to support hESC and hiPSC growth and maintenance, followed by feeder cell-free cultures to create embryoid bodies, then mesenchymal cells, which then were induced to differentiate into functional megakaryocytes and platelets.

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Document Details

Document Type
Technical Report
Publication Date
Oct 15, 2012
Accession Number
ADA566171

Entities

People

  • Kenneth S. Zuckerman

Organizations

  • University of South Florida

Tags

DTIC Thesaurus Topics

  • Blood
  • Blood Cells
  • Blood Coagulation Factors
  • Bone Marrow Cells
  • Cell Physiological Processes
  • Cells
  • Cultured Cells
  • Department Of Defense
  • Erythrocytes
  • Growth Factors
  • Hematology
  • Hematopoietic Cells
  • Law
  • Maintenance
  • Production
  • Stem Cells

Fields of Study

  • Biology

Readers

  • Immunology
  • Immunology and Pathology
  • Molecular and Cellular Biology

Technology Areas

  • Biotechnology