The Snail-Induced Sulfonation Pathway in Breast Cancer Metastasis

Abstract

This projects seeks to establish a key role for cellular sulfonation enzymes in the metastatic progression of breast cancer. To this end we have accomplished the following tasks in this first year of support: 1. Creation Tetracycline inducible WT Snail and mutant Snail vectors for expression in cell lines, 2. Creation of stable cell lines in MCF10A cells and 67NR cells via a lentiviral expression system. 3. Demonstration MCF-10A cells undergo dramatic morphologic EMT in the presence of WT Snail expression, with the downregulation of the Cell adhesion molecule E-cadherin and the upregulation of the mesenchymal markers vimentin and fibronectin. 4. Characterization of antibodies to PAPSS1 and PAPSS2 enzymes. 5. Demonstration that both enzymes are expressed in Human breast cancer cell lines a with a positive correlation to Snail levels. 6. Derivation and characterization of shRNA knockdown vectors for PAPSS1 and PAPSS2.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2012
Accession Number
ADA566631

Entities

People

  • Frank J. Rauscher Iii

Organizations

  • Wistar Institute

Tags

DTIC Thesaurus Topics

  • Acquisition
  • Antibodies
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Demonstrations
  • Gene Expression
  • Genes
  • Genetics
  • Mammary Glands
  • Metastasis
  • Molecules
  • Neoplasms
  • Proteins

Fields of Study

  • Biology

Readers

  • Marine Ecological Systems Migration
  • Molecular Genetics
  • Oncology (Cancer Research).