Microfluidic Flow Retardation Device for Tagless Cancer Cell Analysis for Metastatic Potential
Abstract
We developed a microfluidic device to detect surface protein expression in individual cancer cells in small cell populations without prior labeling. We used LM2 cells (kind gift of Juan Massague, Memorial Sloan-Kettering Cancer Center, NY), derived from murine lung metastases of MDA-MB-231 cells with a unique lung metastatic potential. We selected LM2 cells for 100% sustained expression of IL-13R 2 and cells without expression. We coated the channel with an antibody to IL-13R 2 or with BSA and directed cells over the patch by a microsyringe pump at flow rates of nanoliters per second. The transient interactions with surface ligands resulted in cell flow retardation in cells expressing the cognate surface protein over the antibody-coated patches and rapid, unimpeded flow on BSA or in the absence of the antigen with 100% sensitivity and 100% specificity. We detected and tracked cells with microscope objectives and computer tracking programs. We were able to multiplex 6 surface proteins creating a barcode for their expression in each individual cell in a population. We characterized cells in mixed tumors using needle aspirates. All objectives were achieved.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2012
- Accession Number
- ADA566934
Entities
People
- Rajan Kumar