Principles Governing the Stability and Folding Kinetics of Proteins From Extremophiles

Abstract

The goals attained during this project are: (1) Development of extremely computational methods to study allosteric dynamis in enzymes, molecular motos, and chaperones. (2) Application of these techniques to closed to open transition in the enzyme DHFR. The results were in excellent with experiments. More importantly, our predictions were quantitatively validated in NMR experiments. (2) A fully quantitative simulation of the folding landscape of riboswitches. Remarkably, our predictions were verified in 2011 in single molecule experiments fully three years after the predictions. (3) Identified residues that are responsible for transmitting allosteric signals in motors and chaperones.

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Document Details

Document Type
Technical Report
Publication Date
Jan 25, 2012
Accession Number
ADA567225

Entities

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  • Devarajan Thirumalai

Organizations

  • University of Maryland

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  • Energy and Power Technologies

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