Inhibition of Embryonic Genes to Control Colorectal Cancer Metastasis

Abstract

Embryonic core Transcription Factors (TFs), primarily the retrogene NanogP8, are the master regulators of stem cells in human Colorectal Carcinoma (CRC) and positively control neoplastic progression. The major goals of the application are to demonstrate that NanogP8 is expressed in human colorectal carcinoma (CRC) and that inhibition of expression of NanogP8 by lentiviral (LV) short hairpin (shRNA) will regress established human CRC liver xenografts in preclinical models. These data are necessary as proof of principle before we can progress to using LV shRNA in patients with advanced CRC in early phase trials.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2012
Accession Number
ADA567286

Entities

People

  • John Jessup

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Apoptosis
  • Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Colon Cancer
  • Confocal Microscopy
  • Culture Techniques
  • Gene Expression
  • Genetics
  • Phosphodiesterases
  • Polymerase Chain Reaction
  • Stem Cells
  • Therapy
  • Three Dimensional

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Oncology (Cancer Research).
  • Petroleum Engineering

Technology Areas

  • Biotechnology