Reactivation of Breast Cancer Micrometastases by Senescent Bone Marrow Stroma

Abstract

We developed an in vitro injury model of stroma cultured from bone marrow samples of mice and human donors. We demonstrated that oxidative and hypoxic damage and estrogen deprivation can induce IL-6 and IL-8 export by human female, premenopausal stroma. We adapted a breast cancer cell co-culture model to determine effect of injury on stromal capacity to support dormancy and demonstrated that breast cancer cells induce inflammatory cytokine secretion by stroma. We adapted an osteoclast activation model to determine the capacity of injured stroma to activate osteoclasts. We also adapted a preosteoblast co-culture model to determine the role of osteoblasts in the micrometastatic cell niche. Studies on estrogen replacement and anti-inflammatory treatment will determine reversibility.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2012
Accession Number
ADA567337

Entities

People

  • Robert Wieder

Organizations

  • University of Medicine and Dentistry of New Jersey

Tags

DTIC Thesaurus Topics

  • Bone Marrow
  • Bones
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Culture Media
  • Culture Techniques
  • Cytokines
  • Deprivation
  • Diseases And Disorders
  • Estrogens
  • Indicator Dyes
  • Macrophages
  • Neoplasms
  • Stem Cells
  • Tumor Cell Line

Fields of Study

  • Medicine

Readers

  • Oncology (Cancer Research).