PECAM-1 and Angiogenesis
Abstract
It is hypothesized that during in vivo angiogenesis, endothelial PECAM-1-dependent ligand interactions trigger intracellular signaling cascades that facilitate endothelial cell (EC) motility. Three specific aims were proposed to test this hypothesis: (i) Specific Aim #1: Define the role of SHP-2 in mediating PECAM-1-dependent cell motility; (ii) Specific Aim #2: Characterize the PECAM-1-dependent ligand interactions involved in PECAM-1-mediated cell migration; (iii) Specific Aim #3: Determine the role of endothelial PECAM-1 tyrosine phosphorylation and ligand binding during in vivo angiogenesis. In accomplishing these aims we have (i) begun to define a role for PECAM-1 in the formation of filopodia, (ii) made progress toward generating the various constructs and cellular transfectants required for completion of the first two aims; (iii) further defined a role for PECAM-1 in some of the morphological modeling that occurs during angiogenesis; and (iv) done studies of the role of CD44 in in vivo angiogenesis that have complemented our investigations of PECAM-1. These studies are indicative of the progress we have made and may provide new insights into the treatment of angiogenic processes such as tumor growth and wound healing.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2009
- Accession Number
- ADA567787
Entities
People
- Horace Delisser
Organizations
- University of Pennsylvania