Targeting Cell Surface Proteins in Molecular Photoacoustic Imaging to Detect Ovarian Cancer Early
Abstract
Our idea is to apply a series of novel techniques to identify the reagents needed to move imaging technology forward into the clinic. While molecular imaging strategies are now approaching the resolution required to detect ovarian cancer in an early curable stage, specific imaging probes are not currently available and are urgently needed to realize the potential of imaging for ovarian cancer early detection. To address this challenge we are undertaking a comprehensive proteomic analysis of the cell surface membrane of ovarian cancer cells. We have completed a survey of the cell surface glycoproteome of OVCAR3 cells and serous ovarian cancer cells isolated from ascites using a novel biochemical labeling method that allows for highly selective capture and internal validation of candidate peptides and proteins by LC-MS/MS. To date 411 cell surface proteins have been identified. We are currently annotating these proteins as potential targets for molecular imaging probes evaluating them by IHC. Several promising candidates have been identified.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2012
- Accession Number
- ADA567976
Entities
People
- Charles Drescher
Organizations
- Fred Hutchinson Cancer Center