Heterobivalent Imaging Agents Targeting Prostate Cancer Training
Abstract
To determine the utility of imaging MT-SP1 in cancer, xenografts of different cancer cell lines were evaluated with near-infrared imaging using a fluorescently labeled probe to visualize active MT-SP1. Subsequent in vitro studies demonstrated a correlation between the MT-SP1/HAI-1 mRNA ratio and probe localization to tumor tissue and cell lines. Antibody radiolabeled with singlephoton emitting 111In for computed tomography imaging gave a probe for in vivo MT-SP1 detection and quantitation. Pronounced probe localization was observed in cell line derived xenografts and in a patient-derived xenograft (PDX) model. HAI-1 was found to play an important role in modulating MT-SP1 activity in clonal derived cell lines and xenografts, but not in the PDX model. These data show the value of using PDX models to recreate the intrinsic tumor heterogeneity of human cancers and in the development of probes for the non-invasive imaging of cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2011
- Accession Number
- ADA568076
Entities
People
- Aaron M LeBeau
Organizations
- University of California, San Francisco